Getting to the surface of biology
Apr 22, 2022
2:30PM to 3:30PM
Date(s) - 22/04/2022
2:30 pm - 3:30 pm
A variety of different surfaces (or interfaces) exist in biology. For example, the surface of a biomaterial is the interface between that biomaterial and the biological environment. State-of-the-art instrumentation, experimental protocols and data analysis methods are needed to obtain detailed information about these surface structures and their compositions, along with the biomolecules that attach to these surfaces. Surface analysis tools such as time-of-flight secondary ion mass spectrometry (ToF-SIMS) can provide images of polymer biomaterials, cells and tissues with chemical and molecular specificity. These chemically specific images could revolutionize our understanding of biological processes such as the role of changes in tumor metabolism that affect responses to chemotherapy. Since many biomaterials (e.g. porous polymer scaffolds), cells and tissues are three dimensional constructs, it is of interest to be able to characterize their chemical composition in 3D. However, it is challenging to characterize these topographically complex materials with surface sensitive techniques. With the use of gas cluster ion beams (GCIBs) surface analysis tools such as x-ray photoelectron spectroscopy (XPS) and ToF-SIMS can attain very fine z-resolution (<10 nm) in depth profiles. In this presentation, ToF-SIMS analysis of biological samples from 2D images of tissues to 3D images of explanted porous scaffolds will be presented.
Lara Gamble is currently a Bioengineering Research Associate Professor at the University of Washington (UW) and has been on the faculty at UW since 2004. She is also the Co-Director of the NIH NIBIB funded National ESCA and Surface Analysis Center for Biomedical Problems (NESAC/BIO) and Director of the Molecular Analysis Facility (MAF) at the UW. Lara’s scientific research interests include surface modification and characterization, biomaterials, biosensors, and molecular imaging in 2D and 3D. Lara has over 70 publications and has given many invited talks and tutorials on surface analysis. Dr. Gamble received her Bachelors in Chemistry at UC Santa Cruz and her Ph.D. in Physical Chemistry from the University of Washington. She did her postdoctoral research at NESAC/BIO before taking a position at Zyomyx, Inc., a biotech startup in Fremont, CA. Her time in industry was followed by a slight change of direction to work at the Space Dynamics Laboratory (SDL) in Logon, Utah before eventually returning to NESACBIO at the University of Washington.